http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102706869-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fd97b87aabcb0e9af5daf64d42e733f2 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-76 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-26 |
| filingDate | 2012-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9b4a0b81cdb8ffb47f752466b40803fc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dec18a9a4bb0cbfd0d911b78388e7691 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4bf5a2c44a6fe89025e872f7c993ac4d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_045e08525042cd02ccc547de65a72e3d |
| publicationDate | 2014-09-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102706869-B |
| titleOfInvention | System for amplifying alkaline phosphatase label signal |
| abstract | The invention discloses a system for amplifying an alkaline phosphatase label signal. Alkane, olefin or alkyne alcohol phosphate is used as a substrate and generates free phosphoric acid and corresponding alcoholic compounds under the action of alkaline phosphatase; the alcoholic compounds generate aldehyde or ketone compounds and hydrogen peroxide (H2O2) under the participation of oxygen and under the action of alcohol oxidase; the generated aldehyde or ketone compounds are reduced into alcoholic compounds under the action of alcohol dehydrogenase in the presence of NADH (Nicotinamide Adenine Diuncleotide Hydrogen); and the generated alcoholic compounds continuously participate in a circulating reaction. A large amount of H2O2 which is circularly generated is matched with different substrates to be catalyzed by peroxidase and then the catalyzed substance can be detected by a colorimetric method, an electrochemical process, a chemiluminescence method or a fluorimetric method. Therefore, according to the system, independent alkaline phosphatase or alkaline phosphatase connected with an antigen, a hapten, an antibody, protein, nucleic acid and oligonucleotide can be sensitively detected and the function of amplifying the signal is achieved. The structural formula of the substrate is shown as the description. |
| priorityDate | 2012-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 368.