http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102703500-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0ee33b0e3e7398cf6fc957eeee59d510 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-83 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 |
| filingDate | 2012-05-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d9904b4b852f51f1f540ff3a18a77795 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9bb8bd4ef537615dcc6d3115abe0bbf9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e977c142de67b7de4fcf754c867eb3d9 |
| publicationDate | 2014-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102703500-B |
| titleOfInvention | Method for constructing and expressing micro ribonucleic acid (miRNA) target simulation sequence by using plant virus vector |
| abstract | The invention discloses a method for constructing and expressing a micro ribonucleic acid (miRNA) target simulation sequence by using a plant virus vector. Experiments prove that deoxyribonucleic acid (DNA) molecules which are chemically synthesized and encoded and have the miRNA158 or miRNA172 target simulation sequences are inserted into the plant virus expression vector pTY102 to obtain a recombinant expression vector, the recombinant expression vector is imported into tobaccos leaf, and after three weeks, the phenotypic modulation of tobacco and the raising of the expression of the miRNA target genes can be detected. According to the method, bridge polymerase chain reaction (PCR) is not needed; the method is simple; and by using the characteristics of high expression, wide host range and high speed of spreading in the whole plant of virus, the expression level of plant endogenous miRNA can be reduced quickly and conveniently, and a new way is provided for researching the plant endogenous miRNA function. |
| priorityDate | 2012-05-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 243.