http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102703448-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 |
| filingDate | 2012-05-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102703448-B |
| titleOfInvention | Artificial microRNA is utilized to cultivate method and the special-purpose double-chain RNA thereof of anti-rough dwarf disease Semen Maydis |
| abstract | The invention discloses a kind of method utilizing artificial microRNA to cultivate anti-rough dwarf disease Semen Maydis.First the microRNA that corn clone is endogenous, then with it as template, carries out PCR amplification containing the pair of primers of sequenceA and sequenceB respectively, by the sequence after amplification by enzyme action, connects and is transformed in the plant expression vector with strong promoter；Then this plant expression vector is infected by Agrobacterium or the method for particle gun imports in Semen Maydis, obtain the transgenic corns of anti-MRDV.The present invention cultivates anti-rough dwarf disease corn new germplasm by artificial microRNA technology, it is not easy to miss the target, and safety is high, and disease resistance trait can stablize heredity. |
| priorityDate | 2012-05-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 178.