http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102703424-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 |
| filingDate | 2012-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2015-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102703424-B |
| titleOfInvention | A kind of method of genome of E.coli point mutation of recombined engineering mediation |
| abstract | The present invention relates to a kind of method of the genome of E.coli point mutation based on recombined engineering.Present method be first by overlapping-extending polymerase chain reaction, to obtain two ends be each 50bp homology arm consistent with target site both sides sequence, middle for mutational site, the 30bp homologous fragment consistent with target site downstream sequence, both sides be the modification fragment of the kalamycin resistance gene of two nuclease I-SceI restriction enzyme sites of going back to the nest; Then the fragment between 50bp homology arm is integrated into genome of E.coli, mutational site replaces the target site on genome, obtains kalamycin resistance mutant strain.The expression of the Uromycin induction I-SceI enzyme of heat inactivation subsequently, homologous recombination is there is between catalysis 30bp homologous fragment and target site downstream 30bp, thus 30bp homologous fragment, two I-SceI restriction enzyme sites and kalamycin resistance gene are eliminated, obtain the E. coli mutant strain of origination point sudden change. |
| priorityDate | 2012-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 143.