http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102702341-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6cc1068000ead87aef5e4c7760e073f2 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-48 |
| filingDate | 2012-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-01-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5e41298e0d4ed070a3de35822c4885cd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_35ffe5af0fe0e31f689503a9988ce7a5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dc36a18523052b2bc3bbf74abf042010 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_03696e0987ecd91b9cbd151c83b77ddd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8b35af1110a325a07ee7e25612c8c7aa |
| publicationDate | 2014-01-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102702341-B |
| titleOfInvention | Recombinant human nerve growth factor purifying method based on CHO cell expression system |
| abstract | The invention relates to a recombinant human nerve growth factor purifying method based on a CHO cell expression system. The recombinant human nerve growth factor purifying method based on the CHO cell expression system comprises the following steps: 1), centrifugating supernatant, successively removing living cells and cell fragments, and removing impurities and bacteria by filtering; 2), applying a supernatant sample to an EMD SO3-(M) chromatography column, rinsing unbound protein and impure protein, eluting and collecting a target protein peak; 3), concentrating the protein with ultrafiltration equipment with a molecular weight cutoff of 3K; and 4), applying the sample to Superdex 75-containing chromatography column and collecting the target protein peak, namely a recombinant human nerve growth factor stoste. According to the CHO cell expression system and the recombinant human nerve growth factor purifying method based on the CHO cell expression system, the recombinant human nerve growth factor with the purity greater than 98% and the specific activity higher than the 5* 10 to the power of 5 AU/mg can be prepared; compared with other methods, the recombinant human nerve growth factor purifying method based on the CHO cell expression system has the advantages that the steps are reduced, the production cycle is greatly shortened, and the processing capacity and the yield are improved; therefore, the recombinant human nerve growth factor purifying method based on the CHO cell expression system is application for large-scale production. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-107964037-A |
| priorityDate | 2012-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 97.