http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102680601-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_077f0cd6000d9ec9f2c7d78df4ce23ff |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-14 |
| filingDate | 2012-05-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ed33556ea72a467e4953d99defe5d137 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_412181a94209f52092c6d5f9e4692a17 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_81bf4a705ab3cd48966385ea4d8f918e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a9ee680178ad24d564fa204f34ee4b3b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_618c1e5cdf51c5fe09489be708faa5e4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4d885bbacaeb0546eebce0f708c48753 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0b45a11430fe55cbff3664b682cf29aa |
| publicationDate | 2013-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102680601-B |
| titleOfInvention | High-performance liquid chromatography method for measuring nucleotide in milk powder |
| abstract | The invention relates to a high-performance liquid chromatography method for measuring nucleotide in milk powder, and belongs to the technical field of detection for food additives. The method comprises the following steps of: preparing a contrast solution by using a nucleotide standard substance; weighing a test sample, dissolving the test sample in distilled water through ultrasonic oscillation, so that the nucleotide in the milk powder is dissolved in the distilled water; adjusting the pH value of the test sample solution by hydrogen nitrate until the pH value is 3 to 4, precipitating proteins, filtering and removing the proteins, fixing the volume of a filtrate by using water to obtain a to-be-tested solution; measuring nucleotide chromatographic peak areas in the test solution and the contrast solution by high performance liquid chromatography; and calculating the nucleotide content in the test sample by an external standard method according to the concentration of the nucleotidein the contrast solution, the nucleotide chromatographic peak area in the test solution and the mass of the test sample. The method is simple, convenient to operate and high in precision; proteins can be completely separated, so that interference with the measurement for the nucleotide is eliminated; and a good condition is supplied to measurement of the nucleotide in the test sample. |
| priorityDate | 2011-12-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 38.