http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102676638-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_fa783eb077323e5ca6a1d18544900715 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2011-03-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-06-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0af8f196e38a8106773b5c8163dc9b23 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_57017dd0e64fda0445ab080a4e402b0d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9149bb05540b7756ba536bd0f2d61ab6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_103635a4cf13c331894beb7742625eec |
| publicationDate | 2014-06-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102676638-B |
| titleOfInvention | Method and kit for detecting drug-resistance mutation site of ABL kinase domain of BCR/ABL fusion gene |
| abstract | The invention relates to the biological technical field, which discloses an amplification primer for detecting a drug-resistance mutation site of an ABL kinase domain of a BCR/ABL fusion gene. The upstream primer of the amplification primer has a nucleotide sequence shown in SEQ ID No. 1 or SEQ ID No. 2, and the downstream primer of the amplification primer has a nucleotide sequence shown in SEQ ID No. 3. The invention also discloses a method and kit for detecting a drug-resistance mutation site of an ABL kinase domain of a BCR/ABL fusion gene. The invention amplifies the BCR/ABL fusion gene of patients with chronic myelogenous leukemia (CML) by one-step polymerase chain reaction (PCR) amplification, and carries out sequencing by using a specific sequencing primer; and can detect more than twenty kinds of common drug-resistance mutations of the ABL kinase domain of the BCR/ABL fusion gene in a reaction system, which lowers experiment cost, greatly improves accuracy and sensitivity of gene sequencing results, and has a large-scale application prospect. |
| priorityDate | 2011-03-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 43.