http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102676510-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6eeb7934db01a135e12bd4aacfb85b72 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 |
| filingDate | 2012-05-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b5ea8313c0db508a945c297facd9d741 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_354611daf3954e63c804a5f08690de9f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_379e78b4d8571943f8728c0a261a4fe1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8d33e09dbe62e3aa51b801ee16539a83 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4a7ad968ad5e75d9764874d9c0fcd227 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_db13d015509f7b7c8bebf994f297a444 |
| publicationDate | 2013-12-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102676510-B |
| titleOfInvention | Method for enhancing black streaked dwarf resistance of paddy rice by using artificial microRNA (micro Ribonucleic Acid) and special double chain RNA thereof |
| abstract | The invention discloses a method for enhancing black streaked dwarf resistance of paddy rice by using an artificial microRNA (micro Ribonucleic Acid) and a special double chain RNA thereof. The double chain RNA consists of a sequence A and a sequence B. The method comprises the following steps of: replacing a mature body sequence of an entogenous microRNA of paddy rice and an inverse complementary sequence of a mature body thereof respectively by the sequence A and the sequence B; simultaneously, designing a restriction enzyme cutting site and a protecting basic group on 5' and 3' of the microRNA, and obtaining an artificial microRNA vector through artificial synthesis; and transferring the vector into a plant expression vector with a strong promoter through restriction enzyme ligation, and introducing the plant expression vector into paddy rice to obtain transgenic paddy rice which is resistant to black streaked dwarf. The disease resistance of paddy rice is enhanced through an artificial microRNA technology, target missing is prevented, high safety is achieved, and the disease-resistant property can be inherited stably. |
| priorityDate | 2012-05-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 196.