Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c1e0fc105cdaa6fc6208bcc48eadab50 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2021-6439 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-622 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-92 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-533 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6428 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-582 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K49-0058 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6857 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-533 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-531 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-78 |
filingDate |
2010-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2014-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6e12939c84095393ffbb1b43f5af7943 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5a5a02cd4a0abb649eb8bb7992c28408 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6541458a1f862d20d0d36e53ca8b024f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_77c9cf4ccbcd26426410402705641295 |
publicationDate |
2014-08-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
CN-102667480-B |
titleOfInvention |
Fluoroimmunoasssay method |
abstract |
Disclosed is an immunoassay method which does not require a solid phase immobilization step and a washing step, enables the rapid, simple and quantitative measurement of a substance of interest in a liquid phase, and enables the visualization of an antigen. The concentration of an antigen of interest in a substance to be tested can be measured by carrying out the followings steps (a) to (c) sequentially: (a) bringing an antibody light-chain variable region polypeptide and an antibody heavy-chain variable region polypeptide labeled with a fluorescent dye into contact with the antigen in the substance to be tested in a liquid phase, or bringing an antibody heavy-chain variable region polypeptide and an antibody light-chain variable region polypeptide labeled with a fluorescent dye into contact with the antigen in the substance to be tested in a liquid phase; (b) measuring the fluorescence intensity of the fluorescent dye; and (c) calculating the amount of the antigen contained in the substance to be tested on the basis of the positive correlation between the concentration of the antigen in the liquid phase and the fluorescence intensity of the fluorescent dye. |
priorityDate |
2009-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |