http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102660519-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_70a57471adb10e40785c3c694e731ea8 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-69 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-685 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-885 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-445 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-42 |
| filingDate | 2012-05-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c09f2f102f088ebfbb73dbb9a83c3616 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d18096b9ebf26eb2af4e84b252d20dce http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f816105af672d322a893b66f4a0999e1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_63daa53dfb9b875aa1a20673234a790b |
| publicationDate | 2014-07-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102660519-B |
| titleOfInvention | Method for preparing biological enzyme by utilizing fermentation waste liquid |
| abstract | The invention relates to a method for preparing a biological enzyme by utilizing fermentation waste liquid. The method comprises the following steps of: (1), culturing a microbe in a culture medium for 2-10 days, and separating to obtain the residual fermentation waste liquid; (2), preparing a fermentation culture medium of a biological enzyme producing bacterium by utilizing the residual fermentation waste liquid; (3), inoculating a bacterial strain of the biological enzyme producing the bacterium in a seed culture medium to culture for 24-48 hours in conditions that a temperature is 25-30 DEG C and a rotational speed is 120-250 r/min; (4), transferring 5-10% of inoculum size to the fermentation culture medium to produce an enzyme, wherein 1-10% of inducer is added; and (5), culturing for 4-10 days in the conditions that the temperature is 25-30 DEG C and the rotational speed is 120-250 r/min, filtering, and centrifuging to obtain crude enzyme liquid. By using the method, not only is the production cost of an enzyme preparation reduced, but also the harm of the fermentation waste liquid to the environment is decreased, and the low-carbon and emission-reduction purpose that a fermentation waste is changed from the waste into the valuable and a resource is recycled is effectively realized. |
| priorityDate | 2012-05-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 122.