http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102659937-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_9aa470dd3fb9e94c95c7c632f437074c |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 |
| filingDate | 2012-03-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-01-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d90d788580de4a3a63fe5918e3a3b9ea http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7992aef5b440befeebc5e9dc02ee24a9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1c61788ba92c3ff5e823cf157fe89f3d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f36eea88aa7960b08fd1d71a59a3409c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_59c15cf81931e875af2e713e2c9df6d6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3d118cd5184b98af1477dbbdb3bb4547 |
| publicationDate | 2014-01-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102659937-B |
| titleOfInvention | Preparation method and application of recombinant protein and monoclonal antibody thereof |
| abstract | The invention pertains to the field of the biotechnology. The invention relates to a recombinant protein. The recombinant protein includes two dominant antigenic epitopes of humanized heart type fatty acid binding protein, the amino acid sequence of the recombinant protein is converted into corresponding nucleotide sequence by adopting codon preferred by escherichia coli, chemical synthesis is performed on the nucleotide sequence and recombinant expression vector is constructed, so that protein expression level of the recombinant protein in the escherichia coli is increased. The invention also relates to the preparation of monoclonal antibody of the recombinant protein. Immunization, cell fusion and several rounds of screening are carried out to obtain hybridoma cell strain, the monoclonal antibody is purified and labeled with horse radish peroxidase (HRP), the best matching combination of the monoclonal antibody is determined by ELISA orthogonal experiment, and an early diagnosis application of myocardial infarction is achieved. |
| priorityDate | 2012-03-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 114.