| abstract |
The invention provides a method for preparing enterovirus vaccines, which uses gerbil muscle cells as stroma cells of virus culture. The method uses the gerbil muscle cells as the stroma cells for culturing EV71 or CoxA16 viruses, growth titer of the EV71 or CoxA16 viruses on the cells is high, and liquid collection can be conducted repeatedly. The cells can be performed with passage repeatedly, muscle cell strains can be built for production of vaccines, and consumption of gerbil is saved. The cells are primary cells so that the problems that passage cell deoxyribonucleic acids (DNA) are difficult to process and the like do not exist. The vaccine production process is relatively easy, purification is convenient, and the prepared vaccines are safe and reliable when used for vaccination of infants. |