http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102657149-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0cbd749eb61f56e76c45e6b8351d8062 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N1-02 |
| filingDate | 2012-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1d5ede6ecd0d48128b4fc9d4cad56351 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_202867211e09ba6bb930c1a13a484bf3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e82d037eb2033971cf3974f3370d8a0f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_94b2197e508426ea6566a28cdffa2245 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_44fc4c1b6931a7b9f8456754ce29078f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c124db001d4de8638a4544be64194a96 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f8360aa7fd68e07b3d5bb9d525f6c5dd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_05c4ffc3633f0e9ba457aa44875cdb34 |
| publicationDate | 2013-09-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102657149-B |
| titleOfInvention | Ultra-high activity tubule semen freezing method for sheep |
| abstract | The invention relates to a semen freezing method for sheep, in particular to an ultra-high activity tubule semen freezing method for sheep. The method solves the problems that after the conventional frozen semen of sheep thaws, the motility rate and the activity are low, and the frozen effect is unstable. The method comprises the following steps of: filling fresh semen of sheep into the bottom part of a first centrifuging tube holding cleaning liquid; standing in a CO2 incubator for 30 to 60 minutes, wherein the semen with high activity goes up in supernatant; sucking the supernatant and thenfilling into a second centrifuging tube for centrifugation, wherein the semen with high density and high activity is obtained at the bottom of the second centrifuging tube; and sucking the condensed high-activity semen, adding into a frozen diluent, uniformly mixing and filling in tubules; performing program freezing and storing in liquid nitrogen, and thawing the stored semen before semen deposition. The sheep semen is subjected to high activity screening before freezing according to the method, and the motility rate of the thawing tubule frozen semen of sheep reaches 0.45 to 0.60, requirements on the artificial fertilization of the frozen semen of sheep are fully met, and the method can be widely applied to mass production of sheep. |
| priorityDate | 2012-05-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 206.