http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102653774-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_81d6af637871c95f327e1ea7d2593047 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-867 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-071 |
| filingDate | 2011-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6902267bbfc3dae74ec42b0021783f2e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_929468045f3ed600ce0d63ce3b025c34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a3ede1f626979a4b70133da378b5a117 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_57dc7cba756c94eb71be42f6e4c0f95b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3a6f0b1e1d91e7f07527b43364b928d3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4188ed6a8d077ca89bc0cc69229d6981 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b1c78ceeafd03c70a83c95e6ddec8e05 |
| publicationDate | 2014-12-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102653774-B |
| titleOfInvention | Preparation method of inducible pluripotent stem cell of goat |
| abstract | The invention relates to a preparation method of an inducible pluripotent stem cell of a goat. The preparation method comprises the following steps: A) constructing a lentiviral vector carrying a transcription factor which is selected from Oct4, Sox2, c-Myc, Klf4, Lin28 and Nanog; and B) infecting a goat adult cell by combining the transcription factor with the lentiviral vector prepared in the step A, selecting clone with a shape similar to an embryonic stem cell for subculturing, and preparing the inducible pluripotent stem cell of the goat by screening cell clone in accordance with the characteristics of the embryonic stem cell. According to the preparation method, an optimal culture condition and method of a goat ES (embryonic stem) cell line construction can be established; the inducible pluripotent stem cell of the goat is an excellent vector for goat gene targeting, and the inducible pluripotent stem cell of the goat facilitates the revelation of each gene function and the complex development of the goat. |
| priorityDate | 2011-03-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 236.