http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102649942-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6c6d22af4bf4e1b2205a929549da46d3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7fb3f4984f4f1c896467dfc03bc2ebb9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_341129f103718a874c91299a73a473d5 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-41 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 |
| filingDate | 2011-02-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_92c9a7d23f8e0c316f6f6f21ac2e34cb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_30624110b9a195aabd57fb74ccf7d504 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bbf207c581d54f1549e00b838fe410e1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_630ddc65ffc64b7d2ee226c40a51409a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_793eaf4bfa412c3f023cd9cf6f4f78a7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0f240ba9b177a1476246b8b17a545966 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dde4a09c2a26e56a18113b3106df75c0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4024c6f1dbd95a6e160fc5b05d9a3a72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_387538c7c3c7b9ef0012c5497672ce03 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0e79261ca06cbe47e62bba50ab760cae |
| publicationDate | 2013-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102649942-B |
| titleOfInvention | High-density fermentation process of rhizobium huakuii |
| abstract | The invention belongs to the technical field of agricultural microbiology, and particularly relates to a high-density fermentation process of rhizobium huakuii, which is carried out for obtaining highest biomass, in particular to a fermentation method in which C and N sources different from the original fermentation culture medium are replenished. The biomass of the rhizobium huakuii obtained by the process disclosed by the invention can reach more than 13 billions/mL. According to the process method disclosed by the invention, seeds growing to the late period of logarithm are inoculated into a YM (Yeast Manitol) culture medium, liquid content in a fermentation tank is 40-60%, the inoculum size is 1-6%, the temperature of the tank is controlled to be 26-30 DEG C, the rotate speed is 400-800rpm, the first time of material replenishment is carried out after 24-48 hours of fermentation, the second time of material replenishment is carried out after 48-72 hours of growing, fermentation is continued to 60-84 hours after the second time of material replenishment is completed, and then the fermentation is ended. The replenished material comprises glucose of which the final concentration is 0.5-1.5 g/L, yeast powder of which the final concentration is 1-4g/L and peptone of which the final concentration is 1-4g/L. The process disclosed by the invention is simple, the efficiency is superior to that of the prior art, and the production cost is lower. |
| priorityDate | 2011-02-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 49.