http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102645357-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d6a6f422b091ba12ea61d4adbf1b0e8e |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N1-28 |
| filingDate | 2012-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3b0a92acbd8aa35ced5fee70b0d29ce3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8e1c4e36bb0ee479f3dbd47df8e4ab4c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_db65e69dd1585d9d198f257ff7ae62b1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bf69172785c01078e7e818f43df21e62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5dd3e4aed7f3f37e0e39f43a38150bcc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_418ae8f66121f0fbebe5014f08b96281 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bd11fb95eb441e3f12eb0d5e73020396 |
| publicationDate | 2014-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102645357-B |
| titleOfInvention | Method for preparing spirulina dielectrophoresis analysis protein |
| abstract | The invention relates to a spirulina development and application technology, and the purpose of the invention is to provide a method for preparing spirulina dielectrophoresis analysis protein. The method includes the following steps: spirulina cells are frozen under negative 20 DEG C for 1.5 hours and then thawed under 4 DEG C for 1 hour, and the freezing and melting process is repeated for five times until the spirulina cells are completely broken; Tris-HCL extracting solution is used for carrying out extraction for three times to obtain water-soluble protein, and SDS (sodium dodecyl sulfate) extracting solution is used for extracting water-insoluble protein from precipitate. Compared with the conventional spirulina 2-DE (dielectrophoresis) protein preparation method, the spirulina 2-DE protein preparation method established by the invention, i.e. a step-by-step freeze-thaw wall-breaking purification method, is easy to operate, has the remarkable advantages of convenient and simple operation, simple instruments and equipment, low cost, richer information quantity and the like, and can remarkably increase the information quantity and resolution of a spirulina protein 2-DE map, thus laying a necessary foundation for the subsequent bioinformatic researches, such as a proteomics research. |
| priorityDate | 2012-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 45.