http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102640642-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d6a6f422b091ba12ea61d4adbf1b0e8e |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-34 |
| filingDate | 2012-04-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_714db885da8f47202b33b961bbc5f35f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_64c98bef969ff9197208aa41342db0de http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fad10a55e3f6cb97094fbb7375c4eea8 |
| publicationDate | 2013-11-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102640642-B |
| titleOfInvention | Method for pierce-inoculating geminivirus infectious clone-containing solid colony to plant, and application thereof |
| abstract | The invention discloses a method for pierce-inoculating geminivirus infectious clone-containing solid colony to plant, and an application of the method. The method comprises the steps of coating geminivirus infectious clone-containing Agrobacterium tumefaciens EHA105 on YEP solid culture medium containing 100 mg/L kanamycin and 50 mg/L rifampicin; culturing into colony at 28 DEG C; picking colony with toothpicks; piercing 3-4 points on phloem of plant after three-leaf stage, at position 1-2 cm from root; culturing inoculated plant in a greenhouse; and observing virus symptom. The inoculation method provided by the invention has the advantages of no need for raising Bemisia tabaci, high inoculation efficiency and repeatability, simple and rapid operation, and suitability for large-scale popularization. The method provided by the invention is applicable to 16 kinds of geminivirus with severe breakout in our country. The inoculated plants are in families of Solanaceae, Cucurbitaceae and Convolvulaceae. The method can be used for pathogenicity identification of geminivirus and virus gene function research, anti-geminivirus plant variety determination and its breeding. |
| priorityDate | 2012-04-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 70.