http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102634593-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8c635c92c7be33811e9f9203cec9b760 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7e71d6c43b5b54d28958bf1cfaaad6b6 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2012-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a377794e7a786d3137e3ea292a016f9c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_649a83de2cd38b072dfdf8539245f6c9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1d8dbe99f59de1e22373f847246ae767 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_864bb3e6e01a63784db928720ea147cc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5c3c2353c88d23322b9fad27089364c5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_db345eb6773d6a933b7dc1e45e157310 |
| publicationDate | 2013-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102634593-B |
| titleOfInvention | LAMP (mop-mediated isothermal amplification) detection primer group, kit and method for transgenic maize EVENT98140 and derived varieties thereof |
| abstract | The invention discloses an LAMP (mop-mediated isothermal amplification) detection primer group, kit and method for transgenic maize EVENT98140 and derived varieties thereof. The detection primer group comprises four specific primers. The detection kit comprises primer solution, reaction solution, DNA (deoxyribonucleic acid) polymerase, controls and a color developing agent. The detection method is characterized by comprising the following steps: extracting the DNA of the maize variety to be detected, adopting the four specific primers and the DNA polymerase with strand displacement activity to amplify the sample DNA template at 63-65 DEG C, adding SYBRGreenI to observe color change or observing change of turbidity of the precipitates in a reaction tube with a turbidity meter to judge whether the sample DNA template is amplified and determining whether the maize variety to be detected contains or is the transgenic maize EVENT98140 and derived varieties thereof, wherein the short-time amplification efficiency can reach 109-1010 copies. The detection primer group, kit and method disclosed by the invention have the advantages of quickness, efficiency, simpleness and convenience in operation and identification, high specificity, high sensitivity, suitability for field detection and the like and are suitable for popularization and application. |
| priorityDate | 2012-01-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 42.