http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102634591-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_7e71d6c43b5b54d28958bf1cfaaad6b6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8c635c92c7be33811e9f9203cec9b760 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2012-04-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_799edd43667b539e03d53cef172acc2a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_89e75f40b5d89f2a5332d97356931530 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1d8dbe99f59de1e22373f847246ae767 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_78a0f8b827e68088948e597b78521b53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_77c2a0b1293b7ecda62d8cc367b77d39 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a377794e7a786d3137e3ea292a016f9c |
| publicationDate | 2013-08-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102634591-B |
| titleOfInvention | Genetically modified rice BT63 and LAMP (loop mediated isothermal amplification) detection primer group, detection kit and detection method of derived variety thereof |
| abstract | The invention discloses genetically modified rice BT63, an LAMP (loop mediated isothermal amplification) detection primer group, a detection kit and a detection method, a detection kit and a detection method of the genetically modified rice BT63. The detection primer group comprises four specific primers; the detection kit comprises primer liquid, reaction liquid, DNA (deoxyribonucleic acid) polymerase, a reference and also a color reagent. The detection method comprises the steps that a DNA template of a sample is amplified at 63-65 DEG C by extracting DNA of rice variety of rice to be detected and adopting four specific primers and one DNA polymerase with strand displacement activity, wherein the amplification efficiency can achieve 109-1010 copies in a short time, and the identification is as follows: SYBRGreen I is added to observe the color change or a turbidity meter is utilized for observing the turbidity change of sediments in a reaction tube so as to judge whether amplification is conducted or not, so that whether the rice variety to be detected contains genetically modified rice BT63 and the derived variety thereof or is genetically modified rice BT63 and the derived variety thereof is ensured. The detection method provided by the invention has the advantages of rapidness, high efficiency, easiness and convenience in operation, high specificity, high flexibility, easiness and convenience in identification, and suitability of on-site detection, and is suitable for promotion and application. |
| priorityDate | 2012-01-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 50.