http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102634523-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_948e46e8cd67b7e6a46bf7d399747378 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-465 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-31 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N47-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-76 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01P3-00 |
| filingDate | 2012-03-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-04-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1cd611edbd76e85cb414ff1389380c8a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c2c8b4f6b1c5af0ae2834646ec0060b7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_58b341f94a674cdbdba9f1e44e0fd90a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_61180ca5104f88b87934b73a52346cd5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_798b5e9388c26a88a040ea3dfc45026c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6d2728f1bf57b687f9d6a547c7f278d2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_81f14bc592c062511de419e4d15719dd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f54c284ba7f206fd3beb48ec95e82180 |
| publicationDate | 2014-04-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102634523-B |
| titleOfInvention | Negative control gene of streptomyces roseoflavus as well as preparation method and application thereof |
| abstract | The invention relates to a nsdBmgh gene of streptomyces roseoflavus (Men-myco-93-63) as well as a preparation method and application thereof. The preparation method of the nsdBmgh gene of streptomyces roseoflavus comprises the following steps: automatically designing a primer Ns8F and Ns8R through the sequence of a negative control gene nsdB in the metabolic pathway of sky blue streptomycete A3(2) antibiotic, amplifying genomic DNA of the streptomyces roseoflavus (Men-myco-93-63) to obtain fragments of 1121bp, and then extending the two ends of a target sequence by utilizing a nonspecific primer anchoring PCR(NPA-PCR) method so as to obtain the nsdBmgh gene of the streptomyces roseoflavus (Men-myco-93-63). The functions of the nsdBmgh gene are verified through the gene disruption, and the bacteriostatic activity of the gene disruption mutant strain is obviously enhanced as compared with that of metabolin of original strains. The preparation and the application of the nsdBmgh gene provide an important basis for further researching and verifying the synthetic and metabolic pathway and the adjusting and controlling mechanism of the streptomyces roseoflavus, and lay the foundation for the application of high-yield genetic engineering strains of antibiotics for preventing plant diseases. |
| priorityDate | 2011-04-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 62.