http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102627652-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_aa289e44959d16c8c01d53bd8c21a44e |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07D491-22 |
| filingDate | 2012-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_13dc08ca177e78165a82dcc752cb16ae http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ec861f0fe6a1bd308196c5f8e567daa3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fe45e2be049d0b30b0c127711f9fde84 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_93d8bceac4d7bf1930b0ce372867616b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2b52d69ffaaf6a9b02d949527fe0f611 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2b3a332ccee8166fdb62ee7d18e3cb98 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_52f83a352df2df9a74435d62b9f9f438 |
| publicationDate | 2014-05-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102627652-B |
| titleOfInvention | Deoxytetrodotoxin extraction method |
| abstract | The invention discloses a deoxytetrodotoxin extraction method. The deoxytetrodotoxin extraction method comprises the following steps of dissolving a tetrodotoxin crude product in acetic acid, adding anhydrous ethanol into the solution of the tetrodotoxin crude product, stirring uniformly, carrying out centrifugation to remove insoluble substances and to obtain a tetrodotoxin supernatant, heating the tetrodotoxin supernatant in a closed container containing ether so that tetrodotoxin crystals are precipitated and a mother liquor is obtained, concentrating the mother liquor, adding ammonia water into the concentrated mother liquor to adjust a pH value so that precipitates are separated out, treating the precipitates to obtain deoxytetrodotoxin-containing precipitates, dissolving the deoxytetrodotoxin-containing precipitates in acetic acid or phosphoric acid, carrying out liquid chromatographic separation by a preparative high performance liquid chromatograph, carrying out detection by a differential refraction detector or an ultraviolet detector, collecting a deoxytetrodotoxin fraction according to a peak map, adding the deoxytetrodotoxin fraction into a chromatographic column, carrying out elution by an acetum, collecting a fraction, adjusting a pH value of the fraction by ammonia water so that white solids are precipitated, and treating the white solids to obtain deoxytetrodotoxin crystals. |
| priorityDate | 2012-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 56.