http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102618651-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_12c98277898247facc6b9ffb9ba47d3b |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6816 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6876 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2012-04-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_db1a1ab8ec4904fcea025fd753df47cd http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b84e3b9f24f163af5ab7c90b3ca17828 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_216b637a015dfa6306867489ee9a075b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2e09a15eb1e504967b6106108f3c6a36 |
| publicationDate | 2014-06-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102618651-B |
| titleOfInvention | Omega structure oligonucleotide primer for detecting short chain ribonucleic acid (RNA) and application thereof |
| abstract | The invention discloses an omega structure oligonucleotide primer for detecting short chain RNA. The oligonucleotide primer sequentially comprises a polymerase chain reaction (PCR) primer target area containing 20-30 basic groups, a variable coding area containing 0-50 basic groups, an omega stem-loop, a probe spacer region containing at least one basic group, and a probe region containing 4-11 basic groups from a 5' end to a 3' end. The length of stems of the omega stem-loop is that of 4-12 paired basic groups, and the length of loops of the omega stem-loop is that of 3-20 unpaired basic groups. According to the omega structure oligonucleotide primer for detecting the short chain RNA and an application thereof, starting inside chains and primer dimmer can be avoided, target hybridization accuracy of a probe at the 3' tail end can be increased, transcription specificity and sensitivity can be improved, high reverse transcriptase (RT) efficiency and good specificity can be achieved with few primers, a plurality of primers capable of detecting different targets can be added into a reaction system simultaneously, and PCR resultants obtained from different detected targets can be distinguished simultaneously, therefore multi-channel and high-flux detection is achieved, and detection efficiency is greatly improved. |
| priorityDate | 2012-01-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 60.