http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102618620-B
Outgoing Links
| Predicate | Object |
|---|---|
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 |
| filingDate | 2012-04-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2016-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2016-08-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102618620-B |
| titleOfInvention | A kind of method of cell-based screening metallo-matrix proteases inhibitor |
| abstract | A kind of method that the present invention relates to cell-based screening metallo-matrix proteases inhibitor, including: (1) design primer, the full-length cDNA of PCR amplification metallo-matrix proteases；(2) expression system with Retro X Tet Off as carrier is built；(3) cell line is transfected by the carrier that above-mentioned structure completes, and screening positive clone, at the bottom of cell is paved with ware after, culture medium is changed to the culture medium without DOX, the expression of induction MMPs；(4) metallo-matrix proteases inhibitor is filtered out.The present invention establish one based on Tet off expression system, method that is that can at utmost simulate the environment of MMP, for the screening of MMP inhibitor, relative common metal stromatin enzyme inhibitor in-vitro screening is more accurate. |
| priorityDate | 2012-04-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 14.