http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102618574-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3f1130e23cff1328c0ae14101d12103f |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 |
| filingDate | 2012-04-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-09-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ff5ca5e321eaee1c7c761eecf0c804c8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ba5e618b9c08908c0ac01cbb15aa12dc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8b3a170054bd85d55c39a8aa7660e250 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7ee83e0753230e468f162a6a62859c97 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9abfe80c0af2f36d451ea9439d23f68a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c773231f3ebfb47aae1838cb5e24e9f9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_584100d2dcc324878c9c7bbfbbd2f6bb |
| publicationDate | 2014-09-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102618574-B |
| titleOfInvention | Method for direct genetic transformation of tobacco through vacuum infiltration |
| abstract | The invention discloses a method for direct genetic transformation of tobacco through vacuum infiltration. The method comprises the following steps of: immersing pre-cultured tobacco immature leaf explants in pAHC25 plasmid DNA (Deoxyribonucleic Acid) solution carrying target gene; performing vacuum infiltration treatment; then culturing the leaf explants on a screening culture medium containing phosphinothricin; inducing anti-phosphinothricin resistance calluses formed on the explants to differentiate adventitious buds; continuously growing the adventitious buds to form test-tube plantlets; and identifying a transgenic plant carrying the target gene by using a PCR (Polymerase Chain Reaction) amplification method. By using the technical scheme provided by the invention, the target gene is directly introduced into tobacco cells; compared with agrobacterium tumefaciens-mediated genetic transformation, the measure for suppressing the growth of the agrobacterium tumefaciens is saved; compared with the genetic transformation of chemical methods such as polyethylene glycol, steps of separation and culture from protoplast are saved; and compared with the genetic transformation of physical methods such as gene gun, expensive instrument equipment is not required and the operation technology is simple. |
| priorityDate | 2012-04-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 57.