http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102618465-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f90c1df3857ee72900e0015c75caecd9 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-465 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-20 |
| filingDate | 2012-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-02-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_58a3d1c30f1cee04bd919bdb59a51dd7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_79aec08927ba0641ae1ba026f3b4fed4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d2f69c928b3833438b5471758066d5b9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d2fd45d61de7ae7fe10967c2793d9c18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_64b99240a22930cc9f5ece28743556c6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_42926db401a11c200eb190e59d84c068 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e2871163115a72857882821cac1f047e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_decb6bc04531db93379ea370c474549c |
| publicationDate | 2014-02-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102618465-B |
| titleOfInvention | Streptomyces and application thereof |
| abstract | The invention discloses a streptomyces sp. NK-49 and an application thereof; and the application is a method for fermenting, cultivating, separating and purifying Epsilon-polylysine. The method comprises the following steps: firstly, picking a ring of spores from streptomyces sp. NK-49 which is stored on a Beinart inclined plane, and inoculating the spores serving as seeds in a Gao 1th culture medium for activated culture within 20 to 36 h; inoculating 5 to 15 percent of seed liquid to a synthetic medium taking amylolysis sugar or amylolysis sugar and glycerol as a carbon source, and shaking a flask for fermentation and culture for 48 to 96 h; after fermentation, conducting 6000 to 10000 rpm centrifugation on the fermentation liquid to remove thallus, collecting supernate, regulating the pH of the supernate to 8 by utilizing 5M NaOH solution, and filtering to removing deposited hetero proteins; utilizing weak acid type cation exchange resin to adsorb and remove Epsilon-polylysine molecules from the supernate of the hetero proteins, and washing with distilled water; eluting the adsorbed products by utilizing 0.1 to 0.7 mol/L dilute hydrochloric acid, and utilizing 5M NaOH solution to regulate the pH of eluant to a neutral value; and dialyzing the neutral eluant to remove micro molecular impurities, freezing, and drying to obtain Epsilon-polylysine solid powder. The streptomyces sp. NK-49 obtained through separation has the potential for industrial production of Epsilon-polylysine. |
| priorityDate | 2012-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 41.