| abstract |
The invention discloses a preparation method of a new homologue 2-methyl-actinomycin D of actinomycin D. The structure of the compound is determined through comprehensive analysis of nuclear magnetism and mass spectrometry. With a chemical structure as shown in the following figure, the compound is a new homologue of actinomycin D. The preparation method consists of: taking Streptomyces recifensis CGMCC No.5228 as a producing strain; fermenting the Streptomyces in a liquid medium containing soluble starch so as to obtain a mycelium, a fermentation supernatant and various metabolites; extracting the fermentation supernatant and the mycelium from fermentation with chloroform or ethyl acetate, and carrying out extraction, separation and purification, etc. thus obtaining the new compound with the above structure. And cell determination shows that the compound has anti-tumor cell activity. In the invention, a lead compound for developing new antitumor drugs is provided, and is of important value for developing and utilizing medicinal actinomyces resources in China. |