http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102614504-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4cb989cde3b211be844782c91f52f4dc |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-39 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K39-104 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61P31-04 |
| filingDate | 2012-03-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-12-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7fbaa84ae25ba057215da24b509fe9f2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cb3b581e5bfa32c1dade289d9f1e803c |
| publicationDate | 2013-12-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102614504-B |
| titleOfInvention | Pseudomonas fluorescens recombinant protein vaccine and preparation method thereof |
| abstract | The invention relates to the field of molecular vaccinology, in particular to a pseudomonas fluorescens recombinant protein vaccine and a preparation method thereof. The antigen of the vaccine is shown as the amino acid sequence in the sequence list SEQ ID No. 1. The preparation method comprises the following specific steps of: taking pseudomonas fluorescens TSS1 as a template, using F1/R1 as a primer to perform polymerase chain reaction (PCR) amplification, connecting a PCR product and a carrier p EASY-E2, converting the connecting liquid into escherichia coli DH 5 alpha, and selecting plasmid p E478, wherein the plasmid p E478 converts a recombinant protein containing the amino acids in the sequence list SEQ ID No. 1 and expressed by escherichia coli BL21 (DE 3). The immune protection efficiency of the vaccine to the pseudomonas fluorescens is up to 81 percent. |
| priorityDate | 2012-03-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 30.