| abstract |
The invention discloses a specific monoclonal antibody capable of recognizing sulfonamides. The monoclonal antibody of the invention is secreted by hybridoma 4E5 which is preserved in China Center for Type Culture Collection with the preservation number of CCTCC NO: C201148. The invention also discloses a preparation method of the specific monoclonal antibody, coating antigen and immunogen as well as an enzyme linked immunosorbent assay (ELISA) method and an ELISA kit. Compared with the prior art, the monoclonal antibody prepared according to the invention can recognize various sulfonamides. The ELISA method and the ELISA kit of the invention have the advantages of simpleness, convenience, quickness, sensitivity, accuracy and the like. |