http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102608311-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_18086029ee284d70cc9ce46d117d4ff3 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-544 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-65 |
| filingDate | 2012-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-12-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ca7838ce38061eb7c8be356d48b23871 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_413a3c7e3e7226a0eb79cfebfd6e8fc1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bd717ab42d573343df7b8c7fb477a7e4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bc834269763c3493e1ad78141532cacb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_277b434030e3fc3f3f3a19080b2a2eb9 |
| publicationDate | 2013-12-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102608311-B |
| titleOfInvention | Biological sensing method for cholera toxin based on agglomeration of phospholipid bilayer membrane covered nanoparticles |
| abstract | The invention discloses a biological sensing method for quantitatively analyzing cholera toxin, wherein a phospholipid bilayer membrane covered Au nanoparticle probe modified by Raman dye is prepared; functional phospholipid, namely phospholipid with a modified sulfydryl at the head end thereof, is selected; and the functional phospholipid can be covalently bound to the surface of Au nanoparticles so that the stability of the Au nanoparticle probe is improved. Cholera toxin subunit B (CTB) is specifically bound with a plurality of acceptor GM1 molecules, thus resulting in the agglomeration of Au nanoparticles covered with GM1 molecules; as a result, surface plasmas are coupled, thereby realizing high-sensitivity SERS (Surface Enhanced Raman Scattering) detection. Simultaneously, the method is capable of causing the change of the light absorption property and also capable of performing detection by a colorimetric method. The method is simple and quick in operation steps, free of applying any mark to the substrate, low in cost, high in sensitivity and good in specificity; the method is hopeful to become a useful technical way for detecting or identifying the cholera toxin; and the method plays an important role in monitoring food security, and controlling and preventing food-borne diseases. |
| priorityDate | 2012-04-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 26.