http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102597232-B
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3fdc95d761cd63b4c8861a9ae5926070 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-48 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-80 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
filingDate | 2009-08-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2014-08-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dd8a46ff749ec75c00672e7954aa31ff http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_515c44f1a8208bf9c424f89f8e3c0f26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9bda094f204b063ebad7f6578fe4a94b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1edc0c2cacf1439b7817bb04cebf973d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2b4d4ece2c067363f0d9c944e66595ac |
publicationDate | 2014-08-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-102597232-B |
titleOfInvention | Method for designing mutant enzyme, method for preparing mutant enzyme, and mutant enzyme |
abstract | Disclosed is a novel method for improving an enzyme capable of deamidating a protein. A mutant enzyme can be designed by the following steps (1) and (2): (1) specifying at least one amino acid residue among amino acid residues corresponding to the amino acid residues located at position-35, position-38, position-39, position-40, position-41, position-42, position-43, position-45, position-46, position-49, position-79, position-80, position-81, position-82, position-83, position-84, position-103, position-104, position-105, position-106, position-117, position-142, position-143, position-146, position-166 and position-185 in the amino acid sequence depicted in SEQ ID NO:2 which is an amino acid sequence for a protein deamidase (an enzyme to be mutated); and (2) constructing an amino acid sequence having the substitution of the amino acid residue specified in step (1) by another amino acid residue or having the deletion of the amino acid residue specified in step (1) by using the amino acid sequence for the enzyme to be mutated as a base sequence. |
priorityDate | 2008-09-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1002.