http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102590404-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0cb011d981829bdcc85a40133a181da8 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-06 |
| filingDate | 2011-01-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9b984800a154aab36b8a92e5b7814ea5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_49edba80b416d0d0440de7ddd7f3e6ee http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_45f49ebabaa2a00a633cf70d89a9adaa http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_93d345826b7ac94a07d2e9247c04773b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_45cae8545a590d2f30fbbe9544455e18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_58d3e3cbe661cc44cfe3c64d82ecd9cb |
| publicationDate | 2014-03-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102590404-B |
| titleOfInvention | Method for testing contents of absorbed and transported diterpene alkaloids of radix aconiti carmichaeli in Caco-2 cell model |
| abstract | The invention provides a method for testing the contents of absorbed and transported diterpene alkaloids of radix aconiti carmichaeli in the Caco-2 cell model. The method includes the following steps: (a) the diterpene alkaloids in radix aconiti carmichaeli are extracted, so that alkaloid extract is obtained; (b) the alkaloid extract is dissolved in Hanks balanced salt buffer solution, absorption and transportation are carried out in the Caco-2 cell model by using the obtained solution as a supply side and the Hanks balanced salt buffer solution as a receiving side, and a sample to be tested is obtained from the receiving side; (c) with reserpine as an internal standard, an ultrahigh-performance liquid chromatograph and a mass spectrograph are adopted to separate and test the sample to be tested, so that a mass spectrogram is obtained; (d) according to the mass spectrogram and a preestablished standard working curve, the content of each absorbed and transported alkaloid in the Caco-2 cell model is obtained. The ultrahigh-performance liquid chromatograph has a good separation effect on complex samples, while the mass spectrograph has higher sensitivity and accuracy, therefore the testing result is more accurate. |
| priorityDate | 2011-01-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 50.