http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102586472-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2a5debb4370919f95ca230b2907eb647 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-93 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 |
| filingDate | 2011-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2015-01-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_694d7d843e3f112b27fdf4af3cefe166 |
| publicationDate | 2015-01-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102586472-B |
| titleOfInvention | Method and kit for assaying hepatitis B virus DNA (deoxyribonucleic acid) sequence |
| abstract | The invention relates to the field of genetic engineering, and provides a method and a kit for assaying the hepatitis B virus DNA (deoxyribonucleic acid) sequence. The method includes the following steps: (A) hepatitis B virus specific primers are utilized to amplify a plurality of target regions in a sample to be assayed, and a sequencing library is constructed on the basis of amplification products; (B) the single-molecule amplification of the sequencing library is carried out, so that a plurality of single-molecule amplification products corresponding to the target regions are obtained; (C) the high-throughput gene sequencing of the single-molecule amplification products is carried out simultaneously, so that the sequence information of the target regions is obtained. The kit comprises the hepatitis B virus specific primers and a connector element. The method and the kit can carry out deep region sequencing on a plurality of target regions of hepatitis B virus at the same time, consequently, the efficiency of assay is increased, meanwhile, the accuracy and sensitivity of assay can be increased as well, and furthermore, multi-region sequencing can be carried out on a large quantity of samples at the same time. |
| priorityDate | 2011-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 25.