http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102586436-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4b10aa964c21a57e6056fb836049ca02 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2012-02-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d58c84fbffeb0016918e809019faf2e3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c26fb560e4ad7ac4a845edb3cc0fd007 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dfb3334443fc4517c2998975eb97d42d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9a4ecac2f7165214d12aaa1e1eb051d2 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_44d285a92615095ec243e1ca2e6e8c30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_371e67ebf31094f728afa0a416c5eb95 |
| publicationDate | 2014-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102586436-B |
| titleOfInvention | Loop-mediated isothermal amplification (LAMP) detection method for identifying beef and mutton |
| abstract | The invention discloses a loop-mediated isothermal amplification (LAMP) detection method for identifying beef and mutton, falling into the field of molecular genetics. The method comprises searching in GenBank to obtain cattle species-specific conserved sequence designed primers, and performing specific screening on designed primers by using LAMP real-time turbidometer; preparing LAMP reaction solution to construct detection system; taking 1muL extracted genome DNA and 1muL fluorescence detection reagent, adding into the LAMP reaction solution to perform LAMP, and identifying according to color of reaction solution, wherein if reaction solution turns into green, the sample contains beef or mutton. The method has convenient and precise detection, easy preparation of templates and primers, high specificity and sensitivity, naked-eye observation of detection result, and wide application prospect; and can satisfy detection requirements of each detection department. |
| priorityDate | 2012-02-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 42.