http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102586326-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0f32d9276f6a22ce55ea151bc2e99150 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-85 |
| filingDate | 2012-01-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f9ffc418a5f6be309eef7bf046fa0193 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e4a6f721492761162ed271013f44a609 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_178c54546c117cbafd8a3ae2c9c5acda |
| publicationDate | 2013-05-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102586326-B |
| titleOfInvention | Method for overexpression of porcine circovirus type 2 nucleocapsid protein in cells of mammal |
| abstract | The invention discloses a method for overexpression of porcine circovirus type 2 nucleocapsid protein in cells of a mammal. According to the method disclosed by the invention, the stability is enhanced and the expression level of the virus protein in the cells of the mammal is further improved by inhibiting the ubiquitination of the porcine circovirus type 2 nucleocapsid protein. The method comprises the following steps: 1) cloning the genome sequence of a porcine circovirus type 2 SX04 strain; 2) constructing a eukaryotic expression plasmid containing the porcine circovirus type 2 (PCV2) nucleocapsid protein; 3) performing transfection on porcine kidney cells PK15 through the plasmid, and adding MG132 into a cell culture solution at 24 hours after transfection; and 4) culturing continuously for 48 hours, then collecting the cells and detecting the expression protein by immunoblotting. Compared with an ordinary eukaryotic expression method, the method has the advantage that the PCV2 nucleocapsid protein in the eukaryotic cells can be effectively stabilized. The detection by protein immunoblotting at 72 hours after transfection shows that, through the adoption of the method disclosed by the invention, the expression level of the PCV2 nucleocapsid protein can be improved by about 20 times to the greatest extent. |
| priorityDate | 2012-01-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 73.