http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102586317-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6c6d22af4bf4e1b2205a929549da46d3 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-82 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H4-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2011-01-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-08-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dbedd4ffc78ada0c616627409abb5a62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_92edf020d8ae1d9c0aff4370af941d99 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_750a9aa519a0c27e0ad9aeb09f1a452e |
| publicationDate | 2013-08-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102586317-B |
| titleOfInvention | Method for transforming and cultivating citrus transgenic plant by agrobacterium-mediated leaf |
| abstract | The invention belongs to the field of plant transgenic technique, particularly relates to a cultivating method of a citrus transgenic plant and discloses a method for cultivating the citrus transgenic plant by utilizing agrobacterium mediation and taking a citrus test-tube seedling blade as an explant. The method comprises the following steps of genetic transformation, screening and regeneration, PCR (Polymerase Chain Reaction) detection and GFP (Green Fluorescent Protein) fluorescence observation. The method is characterized in that a GFP reporter gene is guided into the receptor citrus blade through agrobacterium mediation, different factors capable of influencing the blade transformation are screened, kanamycin is utilized as a selector to screen a transformed receptor cell, and the transgenic citrus plant is screened and obtained through the PCR detection, the GFP fluorescence observation and other auxiliary methods. The method provides a new technical platform for innovative germplasm resources of citrus genetic transformation. |
| priorityDate | 2011-01-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 59.