http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102586184-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_af48e545dd864488417944c9181d97e9 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0735 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0775 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G06F19-28 |
| filingDate | 2012-02-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-10-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5a377465e05ab968a789ecfe2fec7f4a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_42fc405ba6298450bd1f24a15cf889be http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8a0298bdb61c57fdc3a86fba6c34954a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3dafc015d736b7965eeadb0d41179537 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c999e86fc36146679bb7c6088571ad4e |
| publicationDate | 2013-10-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102586184-B |
| titleOfInvention | Method for establishing placental mesenchyme stem cell library |
| abstract | The invention relates to a method for establishing a placental mesenchyme stem cell library. The method comprises the steps of digesting placental lobules with PBS buffer solution containing tissue digestive enzyme to separate mononuclear cells; suspending the obtained cells with an MSC (mesenchyme stem cell) culture medium; culturing the cells in a 5% CO2 incubator at 37 DEG C; after disseminated cells form clones, respectively culturing each cloned cells with the MSC culture medium; after cell infusion, digesting passages with pancreatin to obtain placental mesenchyme stem cells; detecting the obtained placental mesenchyme stem cells in at least one of the following items: cell viability, cell contamination, hereditary diseases, HLA-ABC/DR type matching; freezing the passaged mesenchymestem cells in liquid nitrogen; establishing a data base of the mesenchyme stem cells comprising the information mentioned above; and associating the data base with the freezed cells. The mesenchyme stem cells involved in the method of the invention has high purity. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102676451-A |
| priorityDate | 2011-09-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 210.