http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102586181-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d6a6f422b091ba12ea61d4adbf1b0e8e |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-077 |
| filingDate | 2012-02-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e01fc73a9a38fc4fbac2ea4b5b76ceea http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_87aeb55b9f81b4310d90ff75ba3cccba |
| publicationDate | 2013-06-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102586181-B |
| titleOfInvention | Culture method for inducing mesenchymal stem cell to differentiate for forming osteocyte under condition without CO2 |
| abstract | The invention provides a culture method for inducing mesenchymal stem cell to differentiate for forming osteocyte under the condition without CO2. The culture method comprises the following steps of: culturing the third-generation human mesenchymal stem cell under the conditions of 5 percent of CO2 and 37 DEG C and standing overnight; after the cell is completely attached to the wall and grows until 80 percents of cell is fused, replacing an inductive culture medium and culturing under the conditions of 37 DEG C without CO2 for 14-21 days to obtain osteoblast, wherein the inductive culture medium is formed by adding fetal calf serum, penicillin, streptomycin, hexadecadrol, beta-sodium glycerophosphate and vitamin C in a basal medium and is prepared from the following components calculated by final concentration: 0.41g/L of sodium bicarbonate, 1.5g/L of disodium hydrogen phosphate dodecahydrate, 0.07g/L of monopotassium phosphate and 8.17g/L of sodium chloride; and solvent of the inductive culture medium is an L-DMEM cell culture medium free of sodium bicarbonate. According to the novel culture medium formula provided by the invention, the mesenchymal stem cell osteoblast can be normally induced to differentiate; the differentiation effect is favorable, so that the consumption of equipment is reduced; and the novel culture medium formula is suitable for cell culture and research on induced differentiation under the condition without CO2. |
| priorityDate | 2012-02-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 198.