http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102578078-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_cae21683d3d15f8a303985b93a1f4f54 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-079 |
| filingDate | 2012-02-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_79aea9b4e843527bca9c357da135f391 |
| publicationDate | 2013-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102578078-B |
| titleOfInvention | Frozen stock solution for nerve cells and freezing storage method |
| abstract | The invention discloses cell frozen stock solution for freezing and storing nerve cells and a freezing storage method, wherein, the cell frozen stock solution and the freezing storage method are used for freezing and storing the nerve cells, especially primary animal nerve cells that are isolated freshly, such as hippocampus nerve cells. The frozen stock solution is formed by adding basic medium, fetal bovine serum, dimethyl sulfoxide,6-fructose phosphate and flavonoid compounds to the basic medium; 6-fructose phosphate (w/w) achieves the function of improving cell viability (effective cell rate) when the frozen cells are recovered; and in addition, the flavonoid compounds achieve definite oxidation resistance and can prevent free radical from damaging the cells. The cell frozen stock solution and the freezing storage method can improve the survival rate and the cell function of the nerve cells through freezing storage, the survival rate of unfreezing cells can be increased to reach more than 95 percent, the frozen stock solution for the nerve cells can preserve the nerve cells for a long period, the biological activity of the nerve cells can be ensured, and very high utility values can be achieved. |
| priorityDate | 2012-02-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 43.