http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102559897-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3595a26d3f7906b6f21f347786339250 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2012-01-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_82cb4b54009be55c060cb5fa27ab50c1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_dc2253c4e8765ffa7b4704b63d103f69 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e950208f94d6e15ba926aaf3d955b3f4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_21f590cddd9a2a8cad3243d7e9a2470c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a00084c99dadd552bea23ef9101165ce http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5e2c56ea45c539c7ddae236a65c03ebe |
| publicationDate | 2013-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102559897-B |
| titleOfInvention | Primer group for NPT(Noctumal Penile Tumescence)II gene detection, corresponding reagent kit for and use method thereof |
| abstract | The invention discloses a primer group for NPT(Noctumal Penile Tumescence)II gene detection, which consists of an outer primer F3, an outer primer B3, an FIP (Forward Inner Primer) and a BIP (Backward Inner Primer); nucleotide sequences of the outer primer F3, the outer primer B3, the FIP and the BIP are respectively shown by SEQ (Sequence) ID (Identity) No. 1-4 or SEQ ID No. 5-9. The primer group has the beneficial effects that whether a target substance exists or not can be judged according to whether to amplify or not, and the specificity is high. The invention also discloses a reagent kit for detecting a NPTII gene, which comprises two pairs of primers, i.e. an FIP/BIP and an outer primer F3/B3, which takes the NPTII gene as a target gene and is designed based on a loop-mediated isothermal amplification technology. The reagent kit has the beneficial effects that the NPTII gene can be quickly detected, the amplification can be carried out only by one constant temperature, a special reagent or equipment is not required, the detection cost is low, and moreover, the sensitivity and the specificity are high. Meanwhile, the invention also discloses a use method of the reagent kit. |
| priorityDate | 2012-01-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 33.