http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102559868-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_795cb009fbe52eb9be778ed97897534c |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2011-11-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_106de3c840e21e2c69c8efed02f71ec1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cf553ba9e14e18246e778ca5cd014f9f |
| publicationDate | 2014-11-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102559868-B |
| titleOfInvention | Method for qualitative and quantitative detection of multiple target nucleotide sequences with single tube |
| abstract | The invention discloses a method for qualitative and quantitative detection of multiple target nucleotide sequences with a single tube, which comprises the following steps of: 1) selecting a relative conservative area in a target gene sequence to design an universal amplimer; 2) selecting an area with high diversity in primer amplified product to design specific probes capable of identifying various target nucleotide sequences, and at the same time designing multiple detecting passages, and designing to divide the probes into multiple groups, wherein, the probe of each group is provided with a same fluorescent group as a detecting passage, and melting point (Tm) value of every probe is artificially controlled by adjusting length and/or basic components of the probe during design process of the probe so as to obtain appropriate differentials between the Tm value of the detecting probes specific for various target sequences in the same detecting passage; 3) analyzing and identifying the target sequences through melting point curve. Every fluorescent passage within the single tube disclosed by the invention can perform the qualitative detection of multiple target nuclear sequences; as well as can determine copy number of each target nucleotide sequence; and can judge ratios between the target sequences under the condition of coexistence of the multiple target sequences. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104862403-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-104862403-A |
| priorityDate | 2011-11-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 310.