http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102559739-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_89d8e7355750aa815842a3fa5b382c19 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-84 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-19 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-19 |
| filingDate | 2012-01-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_729fa87c1f67b2c06cfcf2c28080c3ee http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0d38067a527e2149254edc311a1315a1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2d2544a15acf9bb358b06ed095678986 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a259e92c7bdced68b65cdfe7c3e01c55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_56a6487549193c2db4d984fe6a86687e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9a2965c362aba745835cf996212f296c |
| publicationDate | 2014-06-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102559739-B |
| titleOfInvention | Expressing and purifying method of recombinant human-derived LECT2 protein in Pichia pastoris |
| abstract | The invention discloses an expressing and purifying method of a recombinant human-derived LECT2 protein in Pichia pastoris, and the method is characterized by comprising the following steps of: inserting an artificially synthesized human-derived LECT2 gene into an extracellular expression vector pPICZalphaA to construct a recombinant expression plasmid; carrying out enzyme digestion and linearization, then carrying out electric shock, and introducing the recombinant expression plasmid to Pichia pastoris X33 to obtain a recombinant engineering gene; carrying out shake flask fermentation and methanol induction to realize secretory expression of the recombinant human-derived LECT2 protein; and then, purifying by utilizing column chromatography to prepare the recombinant human-derived LECT2 protein with the purity of over 95%. The expressing and purifying method of the recombinant human-derived LECT2 protein in Pichia pastoris has the advantages that the secretory expressing and purifying method of the recombinant human-derived LECT2 protein in Pichia pastoris is firstly proposed, a great number of recombinant human-derived LECT2 proteins with high activity are expressed by using the Pichia pastoris, and the expressing and purifying method has the advantages of stability, high yield, high activity and the like and can be used for pharmacy and diagnosis detection. |
| priorityDate | 2012-01-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 51.