Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_88a41ec56a4e5d0465902cafea58bd04 |
classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-89 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
filingDate |
2011-06-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate |
2013-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7ff160e1fa29f1d3f8fe32e05ef02f93 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_57f3c39141de3c39a5c8854d5a298f57 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7df6d6832ee67578cb8876f46c7166cb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6d9f90256c551b64f16e5cf96fc0a4e0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d1c97cd028bc3edb0a2d1cf13ebcd86a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_44dd572b798c8cd0055a752cde4e793a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5b12f815c38bf4b7220dec97a32d1581 |
publicationDate |
2013-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
CN-102559710-B |
titleOfInvention |
Isochrysis sphaerica delta 4-fatty acid desaturase gene and cloning method thereof |
abstract |
The invention relates to a delta 4-fatty acid desaturase gene originated from marine microalgae isochrysis sphaerica and a cloning method thereof. The full-length delta 4-fatty acid desaturase gene isfad 4 is obtained by the following steps of: taking the isochrysis sphaerica as a material; designing degenerate primers and amplifying a gene core sequence; and amplifying the full-length delta 4-fatty acid desaturase gene isfad 4 by using a genome walking method. Heterologous expression of the isfad 4 in pichia pastoris proves the 4-fatty acid desaturase function of IsFAD 4. |
priorityDate |
2011-06-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |