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filingDate 2011-06-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
grantDate 2013-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7ff160e1fa29f1d3f8fe32e05ef02f93
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publicationDate 2013-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-102559710-B
titleOfInvention Isochrysis sphaerica delta 4-fatty acid desaturase gene and cloning method thereof
abstract The invention relates to a delta 4-fatty acid desaturase gene originated from marine microalgae isochrysis sphaerica and a cloning method thereof. The full-length delta 4-fatty acid desaturase gene isfad 4 is obtained by the following steps of: taking the isochrysis sphaerica as a material; designing degenerate primers and amplifying a gene core sequence; and amplifying the full-length delta 4-fatty acid desaturase gene isfad 4 by using a genome walking method. Heterologous expression of the isfad 4 in pichia pastoris proves the 4-fatty acid desaturase function of IsFAD 4.
priorityDate 2011-06-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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