http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102558601-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5aa9fbf687ced7d46aeca9aee02ddcc8 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08L5-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08J9-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08J9-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08J3-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08J3-24 |
| filingDate | 2012-01-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d1184ae12e8b665adbdf3c892619db3e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9b8cbc88812f6cfa419740bdf8c78d52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_93c64625ae39c083331c3db13ad7c221 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_70069ab7289de29c32ca0b813d59bc73 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cbec7ac6c5fd5f0c57667322a595cd4f |
| publicationDate | 2013-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102558601-B |
| titleOfInvention | Preparation method of high-strength porous chitosan microcarrier |
| abstract | The invention discloses a preparation method of a high-strength porous chitosan microcarrier. The method comprises the following steps: manufacturing holes by using silica gel, wherein chitosan serves as a raw material; preparing chitosan microspheres containing the silica gel by using a high-voltage pulse microcapsule molding instrument under the high-voltage electrostatic action; precipitating the chitosan microspheres containing the silica gel in sodium polyphosphate solution for 5 hours; washing with distilled water; soaking the chitosan microspheres containing the silica gel into a sodium hydroxide solution at the temperature of 80 DEG C; dissolving the silica gel of the chitosan microspheres containing the silica gel; washing with deionized water; freeze-drying to obtain the porous chitosan microcarrier; and performing microwave radiation treatment to finally obtain the high-strength porous chitosan microcarrier. According to the high-strength porous chitosan microcarrier, the grain size is 400 to 700 microns, the aperture is 20 to 60 microns and the porosity is 93 percent. The high-strength porous chitosan microcarrier can be applied to suspension culture of adherent cells.The preparation method has the characteristics of simplicity and quickness in operation, low cost and good effect. |
| priorityDate | 2012-01-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 33.