http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102533954-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_767b2f19da181d5fb2de3b35f3d7956d |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2010-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-01-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2b39565d6b937a586bd09fad4217ebe7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_391700df16e5193f5aeca59126da8cc5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5155ebc5c242572e3db0edd71f728e9c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f0343f4441576c84a8a218105bedfeb1 |
| publicationDate | 2014-01-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102533954-B |
| titleOfInvention | CYP17A1 (Cytochrome P450) genetic polymorphism detection specific primer and liquid-phase chip |
| abstract | The invention discloses a CYP17A1 (Cytochrome P450) genetic polymorphism detection specific primer and liquid-phase chip. The liquid-phase chip mainly comprises an ASPE (Allele Specific Primer Extension) primer, microspheres and an amplification primer, wherein the ASPE primer consists of a tag sequence at 5' end and specific primers aiming at target gene mutation at 3' end, wherein the specific primers are SEQ ID NO. 7 and SEQ ID NO. 8 aiming at an A221G site, SEQ ID NO. 9 and SEQ ID NO. 10 aiming at C193T, and/or SEQ ID NO. 11 and SEQ ID NO. 12 aiming at T81C; and the microspheres are coated by anti-tag sequence. The matching ratio of the detection result of the CYP17A1 genetic polymorphism detection liquid-phase chip provided by the invention and that of a sequencing method reaches up to 100 percent. The prepared liquid-phase chip has excellent signal-noise ratio; and cross reaction can be avoided and parallel detection of a plurality of polymorphic sites can be realized through selection of a tag label sequence and an anti-tag label sequence and combination of the tag label sequence and a specific ASPE primer. |
| priorityDate | 2010-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 274.