http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102533953-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_767b2f19da181d5fb2de3b35f3d7956d |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2010-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-01-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_979913df0937e4c95137c3e067835070 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f0343f4441576c84a8a218105bedfeb1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5155ebc5c242572e3db0edd71f728e9c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ee4d6ccc1123adb11bb3e9b4281fb8ed |
| publicationDate | 2014-01-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102533953-B |
| titleOfInvention | Specific primers and liquid-phase chip for SNP (Single Nucleotide Polymorphism) detection of LPL gene |
| abstract | The invention discloses specific primers and a liquid-phase chip for SNP (Single Nucleotide Polymorphism) detection of an LPL gene. The liquid-phase chip comprises an ASPE primer, anti-tag sequence coated microspheres, and an amplification primer, wherein the ASPF primer is composed of a 5'-terminal tag sequence and 3'-terminal specific primers for target gene mutation, and the specific primers are respectively SEQ ID NO. 9 and SEQ ID NO. 10 specific for a T161G SNP site, SEQ ID NO. 11 and SEQ ID NO. 12 specific for a C120G SNP site, SEQ ID NO. 13 and SEQ ID NO. 14 specific for a G113A SNP site, and/or SEQ ID NO. 15 and SEQ ID NO. 16 specific for an A178G SNP site. According to the invention, the coincidence rate between the detection result of the liquid-phase chip for SNP detection of the LPL gene and the detection result of a sequencing method is up to 100%, not only is the condition of single site mutation detected, but also the polymorphism conditions of multiple mutant sites can be simultaneously detected in parallel, and the detection effects are consistent. |
| priorityDate | 2010-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 45.