http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102533948-B
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_767b2f19da181d5fb2de3b35f3d7956d |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 2010-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2014-01-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ee4d6ccc1123adb11bb3e9b4281fb8ed http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f0343f4441576c84a8a218105bedfeb1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5155ebc5c242572e3db0edd71f728e9c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_391700df16e5193f5aeca59126da8cc5 |
publicationDate | 2014-01-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-102533948-B |
titleOfInvention | apoB (apolipoprotein B) gene SNP (Single Nucleotide Polymorphism) detection specific primer and liquid-phase chip |
abstract | The invention discloses an apoB gene SNP (Single Nucleotide Polymorphism) detection specific primer and liquid-phase chip. The liquid-phase chip comprises an ASPE (Allele Specific Primer Extension) primer, microspheres and an amplification primer, wherein the ASPE primer consists of a tag sequence at 5' end and specific primers aiming at target gene mutation at 3' end, wherein the specific primers are SEQ ID NO. 12 and SEQ ID NO. 13 aiming at a C181T SNP site, SEQ ID NO. 14 and SEQ ID NO. 15 aiming at a C245A SNP site, SEQ ID NO. 14 and SEQ ID NO. 15 aiming at a C245T SNP site, SEQ ID NO. 17 and SEQ ID NO. 18 aiming at a G153A SNP site, SEQ ID NO. 19 and SEQ ID NO. 20 aiming at a G82A SNP site, and/or SEQ ID NO. 21 and SEQ ID NO. 22 aiming at a G62A SNP site; and the microspheres are coated by anti-tag sequence. The matching ratio of the detection result of the apoB gene SNP detection liquid-phase chip provided by the invention and that of a sequencing method reaches up to 100 percent; and parallel detection of a plurality of polymorphic sites is realized. |
priorityDate | 2010-12-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 45.