http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102533749-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_1ce61bf06f294f2f466c067ed41cc8ce |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01H5-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-66 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-63 |
| filingDate | 2012-02-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-04-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3340711790f22fd956ba2c88dbb60c2f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d2e67d35a1b05cc56c6b1f3fe0a9ddbb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_401b4a4ec784a43d23d193b5095b0485 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c864669873dacbfffefef92d17ad6f46 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_754df876711a329fe3f4c046b348df73 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7f3622069e7ec303b644ff429ecbf114 |
| publicationDate | 2014-04-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102533749-B |
| titleOfInvention | ntCre/LoxP deletion system controlled by heat shock and tetracycline, recombinant expression vector, and preparation method and application of recombinant expression vector |
| abstract | The invention discloses an ntCre/LoxP deletion system controlled by heat shock and tetracycline. The ntCre/LoxP deletion system comprises 2 LoxP loci specifically identified by ntCre recombinase and a target gene part positioned between the 2 LoxP loci, wherein the 2 LoxP loci are respectively a first LoxP locus positioned at the 3' end of target gene and a second LoxP locus positioned at the 5' end of the target gene; and the ntCre/LoxP deletion system also comprises an Rhsf gene expression box controlled by an HSF70m promoter and an ntCre recombinase gene expression box part controlled by an Om35S promoter, wherein the Om35S promoter is controlled by Rhsf gene. The deletion system is controlled by the heat shock and the tetracycline, and the target gene between the 2 LoxP loci can be deleted through heat shock under the condition of no tetracycline. The invention also discloses a recombinant expression vector containing the ntCre/LoxP deletion system. The recombinant expression vector can be applied to deleting target genes in plant transgenes, and has the advantages of short deletion period, simple operation and the like. |
| priorityDate | 2012-02-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 89.