http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102533651-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_e89c206df73c7928c3fa05f87f1287de |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0786 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 |
| filingDate | 2012-01-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-05-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2ce4a928b1a88370b9d3ec4d82aa4d16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d4bfe775b7d18adc42ebdec627ca1844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_699388cfcddbe194544a2e8fad9b380f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_09a60a9b1c029ec32093603829a16cad http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_14132762b19f937d5483cc32e6dda423 |
| publicationDate | 2013-05-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102533651-B |
| titleOfInvention | Pseudosciaena crocea head kidney macrophage separation and primary culture method and application thereof |
| abstract | Provided is technology of external model construction of pseudosciaena crocea head kidney macrophage. The fish head kidney macrophage is horizontally and centrifugally separated by utilizing Percoll with 31%/45% gradient and is cultured in an L15 compound culture medium under the condition that the temperature is 22 DEG C. Each liter of the culture medium contains 50ml fetal calf serum, 0.1 million UI penicillin/streptomycin, 0.05 million UI heparin, 10mmol 14-ethoxyl-piperazine ethanesulfonic acid (HEPES) and 0.5g glucose. The pH of the culture medium is 7.4. The method and the application have the advantages that (1) the proportion of the pseudosciaena crocea head kidney macrophage which is centrifugally separated by utilizing Percoll with 31%/45% gradient in the separation cell is as high as 42%; (2) the purity of the adherence pseudosciaena crocea head kidney macrophage after liquid culture is as high as 90%; (3) the livability of the pseudosciaena crocea head kidney macrophage after one week culture is higher than 58%; and (4) a function checking experiment proves that LPS can remarkably affect the swallow function and breath eruption activity of the cultured pseudosciaena crocea head kidney macrophage. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103468636-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-103468636-A |
| priorityDate | 2012-01-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 34.