http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102532584-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5aa9fbf687ced7d46aeca9aee02ddcc8 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08J9-28 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08L5-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M3-00 |
| filingDate | 2012-01-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d1184ae12e8b665adbdf3c892619db3e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6af8f8f2cd2480f4204664629d06f8ad http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cbec7ac6c5fd5f0c57667322a595cd4f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_93c64625ae39c083331c3db13ad7c221 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ab60a2f7c3d273c821e0d346cd176e03 |
| publicationDate | 2013-09-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102532584-B |
| titleOfInvention | Method for preparing porous three-dimensional chitosan scaffold |
| abstract | The invention discloses a method for preparing a porous three-dimensional chitosan scaffold, wherein chitosan is taken as a raw material, ammonium acetate and n-amyl alcohol are taken as pore-foaming agents, and an emulsion freeze-drying method is adopted to prepare the porous three-dimensional chitosan scaffold. The method for preparing the porous three-dimensional chitosan scaffold comprises the steps as follows: a chitosan acid solution with certain ammonium acetate is prepared and is stirred for 3 minutes, then 10ml of n-amyl alcohol is added, and the solution is continuously stirred for 10 minutes; the obtained emulsion is poured into a die for freeze-drying, and then the freeze-dried scaffold is dried for 35 minutes at the high temperature of 96 DEG C; and finally, the dried scaffold is soaked in 70 percent of alcohol for 24 hours, so that the three-dimensional chitosan scaffold is obtained, wherein the pore diameter ranges from 10 to 60 micrometers, the porosity factor is 93 percent, and the connectivity between holes is good. The obtained porous three-dimensional chitosan scaffold can be used in a technical research on cell culture. The method for preparing the porous three-dimensional chitosan scaffold is simple to operate and achieves compact technology. |
| priorityDate | 2012-01-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 35.