http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-102532408-B
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f90c1df3857ee72900e0015c75caecd9 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08F220-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08F220-56 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08F220-60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08J9-26 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08F2-44 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08F222-38 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08K9-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08K9-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C08K3-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B82Y40-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C01G49-06 |
| filingDate | 2011-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2014-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1fdd41f7a2d51d31674069e131f477fb http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_09687b6473b9e30ee6c79f28cd518e03 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f758cf894b8ef8cf77b81963f6d7f182 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_861b8a4df3700e824d4b6994ba2262d7 |
| publicationDate | 2014-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-102532408-B |
| titleOfInvention | Preparation method of temperature sensitive magnetic western-blotting nanosphere |
| abstract | A preparation method of temperature sensitive magnetic western-blotting nanospheres comprises the following steps: synthesizing magnetic nanospheres with a particle size of 200 nm by a hydrothermal method; coating a silicon layer with a thickness of 20 nm on the surfaces of the magnetic nanospheres by a gel-sol method; adding the silicon-coated magnetic nanospheres, functional monomers, template molecules, and a cross-linking agent into a Tris-HCl buffer to obtain a reaction solution; performing polymerization of the reaction solution at a certain temperature to obtain polymers; eluting the polymers by an eluate to obtain the temperature sensitive magnetic western-blotting nanospheres. The advantages of the invention are that: the temperature sensitive magnetic western-blotting nanospheres combine the specific recognition of western blotting, the rapid separation performance of magnetic nanospheres in an external magnetic field, and the temperature responsibility of temperature sensitive materials; the preparation method is simple; the conditions are mild; the price is low; and a new feasible method is provided for the selective removal and enrichment of high-abundance protein components in a complex biological system. |
| priorityDate | 2011-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 52.